Essential Question

What is the most important thing to know in the diagnosis of infectious disease?
Victoria M.
North House
Senior Topic: Infectious Diseases

Thursday, November 10, 2011

Science Fair Proposal- Growing Bacteria

  1.  If a keyboard is used for a general day then the amount of bacteria on it will be exponentially high.
  2. My dependent is the bacteria growth, my independent variable is the days, and my controlled are the keyboards.
  3. The connection that my science experiment with my EQ, is that it kind of is one of the answers to my EQ, What is the most important thing to know in the diagnoses an infectious disease? In my interview with Dr. Chase, I asked him what skills are needed in being an Infectious Disease Doctor. He responded that you should have a varied knowledge in bacteria, viruses, biochemistry, organic chemistry and anatomy. Calculating the bacterial growth, can help me learn a lot about how a bacteria colony works. 
  4.  Materials:
  •  80 Plastic Petri Dishes & covers, 90 x 16 mm.
  • 8 Agar Nutrients Bottles (125 ml)
  • 80 Cotton Swabs
  • Disinfecting Wipes
  • 10 Keyboards
  • Tape
  • 80 Zip Lock Bags
  • I will come in the 1st Monday morning and I will disinfect every keyboard in Ms. Melagno's class. Then I will come back Tuesday morning to collect samples, and prepare the Petri dishes. I will get my bottle of Agar Nutrients and cover the Petri dish with it, then I use the top half of the Petri dish to loosely cover the bottom and allow the solution to cool and harden for at least an hour. I will roll the clean cotton swab over the keyboards, then swiggle them on the Agar gel. To get a better sample, I will dip the cotton swab in water. Cover the Petri dish with the top half and use a piece of  tape to label the dish with "Keyboard #". Then I will place the sealed Petri dish inside a zipper-lock bag and seal it closed.  I will place the plates in a warm dark place to grow. From then on I will record the growth of the bacteria on Tuesdays and Thursdays. I will be doing this experiment over and over again for about 3 weeks.
5. My category will be Animal Biology and Physiology.